Please use this identifier to cite or link to this item:
http://hdl.handle.net/20.500.11861/7521
Title: | Apoptosis induced by 1,3,6,7-tetrahydroxyxanthone in Hepatocellular carcinoma and proteomic analysis |
Authors: | Fu, Wei-Ming Zhang, Jin-Fang Wang, Hua Tan, Hong-Sheng Wang, Wei-Mao Chen, Shih-Chi Zhu, Xiao Chan, Tak-Ming Tse, Ching-Man Prof. LEUNG Kwong Sak Lu, Gang Xu, Hong-Xi |
Issue Date: | 2012 |
Source: | Apoptosis , 2012, vol. 17(8), pp. 842-851 |
Journal: | Apoptosis |
Abstract: | Gamboge is a traditional Chinese medicine and our previous study showed that gambogic acid and gambogenic acid suppress the proliferation of HCC cells. In the present study, another active component, 1,3,6,7-tetrahydroxyxanthone (TTA), was identified to effectively suppress HCC cell growth. In addition, our Hoechst-PI staining and flow cytometry analyses indicated that TTA induced apoptosis in HCC cells. In order to identify the targets of TTA in HCC cells, a two-dimensional gel electrophoresis was performed, and proteins in different expressions were identified by MALDA-TOF MS and MS/ MS analyses. In summary, eighteen proteins with different expressions were identified in which twelve were up-regulated and six were down-regulated. Among them, the four most distinctively expressed proteins were further studied and validated by western blotting. The b-tubulin and translationally controlled tumor protein were decreased while the 14-3-3r and P16 protein expressions were up-regulated. In addition, TTA suppressed tumorigenesis partially through P16-pRb signaling. 14-3-3r silence reversed the suppressive effect of cell growth and apoptosis induced by introducing TTA. In conclusion, TTA effectively suppressed cell growth through, at least partially, up-regulation of P16 and 14-3-3r. © Springer Science+Business Media, LLC 2012. |
Type: | Peer Reviewed Journal Article |
URI: | http://hdl.handle.net/20.500.11861/7521 |
ISSN: | 1573675X |
DOI: | 10.1007/s10495-012-0729-y |
Appears in Collections: | Applied Data Science - Publication |
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