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Title: Apoptosis induced by 1,3,6,7-tetrahydroxyxanthone in Hepatocellular carcinoma and proteomic analysis
Authors: Fu, Wei-Ming 
Zhang, Jin-Fang 
Wang, Hua 
Tan, Hong-Sheng 
Wang, Wei-Mao 
Chen, Shih-Chi 
Zhu, Xiao 
Chan, Tak-Ming 
Tse, Ching-Man 
Prof. LEUNG Kwong Sak 
Lu, Gang 
Xu, Hong-Xi 
Issue Date: 2012
Source: Apoptosis , 2012, vol. 17(8), pp. 842-851
Journal: Apoptosis 
Abstract: Gamboge is a traditional Chinese medicine and our previous study showed that gambogic acid and gambogenic acid suppress the proliferation of HCC cells. In the present study, another active component, 1,3,6,7-tetrahydroxyxanthone (TTA), was identified to effectively suppress HCC cell growth. In addition, our Hoechst-PI staining and flow cytometry analyses indicated that TTA induced apoptosis in HCC cells. In order to identify the targets of TTA in HCC cells, a two-dimensional gel electrophoresis was performed, and proteins in different expressions were identified by MALDA-TOF MS and MS/ MS analyses. In summary, eighteen proteins with different expressions were identified in which twelve were up-regulated and six were down-regulated. Among them, the four most distinctively expressed proteins were further studied and validated by western blotting. The b-tubulin and translationally controlled tumor protein were decreased while the 14-3-3r and P16 protein expressions were up-regulated. In addition, TTA suppressed tumorigenesis partially through P16-pRb signaling. 14-3-3r silence reversed the suppressive effect of cell growth and apoptosis induced by introducing TTA. In conclusion, TTA effectively suppressed cell growth through, at least partially, up-regulation of P16 and 14-3-3r. © Springer Science+Business Media, LLC 2012.
Type: Peer Reviewed Journal Article
ISSN: 1573675X
DOI: 10.1007/s10495-012-0729-y
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