Please use this identifier to cite or link to this item: http://hdl.handle.net/20.500.11861/7520
Title: Heat shock protein 27 mediates the effect of 1,3,5-trihydroxy-13,13-dimethyl-2H-pyran [7,6-b] xanthone on mitochondrial apoptosis in hepatocellular carcinoma
Authors: Fu, Wei-ming 
Zhang, Jin-fang 
Wang, Hua 
Xi, Zhi-chao 
Wang, Wei-mao 
Zhuang, Peng 
Zhu, Xiao 
Chen, Shih-chi 
Chan, Tak-ming 
Prof. LEUNG Kwong Sak 
Lu, Gang 
Xu, Hong-Xi 
Issue Date: 2012
Publisher: Elsevier B.V.
Source: Journal of Proteomics , 2012, 75(15), pp. 4833-4843
Journal: Journal of Proteomics 
Abstract: Hepatocellular carcinoma (HCC) is a global public health problem which causes approximately 500,000 deaths annually. Considering that the limited therapeutic options for HCC, novel therapeutic targets and drugs are urgently needed. In this study, we discovered that 1,3,5-trihydroxy-13,13-dimethyl-2H-pyran [7,6-b] xanthone (TDP), isolated from the traditional Chinese medicinal herb, Garcinia oblongifolia, effectively inhibited cell growth and induced the caspase-dependent mitochondrial apoptosis in HCC. A two-dimensional gel electrophoresis and mass spectrometry-based comparative proteomics were performed to find the molecular targets of TDP in HCC cells. Eighteen proteins were identified as differently expressed, with Hsp27 protein being one of the most significantly down-regulated proteins induced by TDP. In addition, the following gain- and loss-of-function studies indicated that Hsp27 mediates mitochondrial apoptosis induced by TDP. Furthermore, a nude mice model also demonstrated the suppressive effect of TDP on HCC. Our study suggests that TDP plays apoptosis-inducing roles by strongly suppressing the Hsp27 expression that is specifically associated with the mitochondrial death of the caspase-dependent pathway. In conclusion, TDP may be a potential anti-cancer drug candidate, especially to cancers with an abnormally high expression of Hsp27. © 2012 Elsevier B.V.
Type: Peer Reviewed Journal Article
URI: http://hdl.handle.net/20.500.11861/7520
ISSN: 18743919
DOI: 10.1016/j.jprot.2012.05.032
Appears in Collections:Applied Data Science - Publication

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