Chan, Henry L. Y.Henry L. Y.ChanTsui, Stephen K. W.Stephen K. W.TsuiTse, Chi-HangChi-HangTseNg, Eddie Y. T.Eddie Y. T.NgAu, Thomas C. C.Thomas C. C.AuYuen, LillyLillyYuenBartholomeusz, AngelineAngelineBartholomeuszProf. LEUNG Kwong SakLee, Kin-HongKin-HongLeeLocarnini, StephenStephenLocarniniSung, Joseph J. Y.Joseph J. Y.Sung2023-03-272023-03-272005Journal of Infectious Diseases, 2005, Vol. 191 (12), pp. 2022 - 203200221899http://hdl.handle.net/20.500.11861/7601Background. We aimed to investigate the characteristics of hepatitis B virus (HBV) genotype C subgroups in Hong Kong and their relationship with HBV genotype C in other parts of Asia. Methods. Full-genome nucleotide sequences of 49 HBV genotype C isolates from Chinese patients with chronic hepatitis B were compared with the sequences of 69 HBV genotype C isolates and 12 non-genotype C isolates in the GenBank database. Phylogenetic analysis was performed to define the subgroups of HBV genotype C on the basis of >4% heterogeneity of the entire HBV genome. Results. HBV in 80% of patients in Hong Kong belonged to a subgroup predominantly found in Southeast Asia (Vietnam, Thailand, Myanmar, and southern China) designated as HBV genotype "Cs," and HBV in the remaining 20% of patients belonged to another subgroup, predominantly found in the Far East (Korea, Japan, and northern China), designated as HBV genotype "Ce." Overall, the mean ± SD nucleotide sequence difference between HBV genotype Cs and HBV genotype Ce was 4.2% ± 0.3%. When HBV genotype Cs and HBV genotype Ce were compared among patients in Hong Kong, HBV genotype Cs was associated with a higher tendency to develop basal core promoter mutations (80% vs. 50%; P = .14), a higher prevalence of C at nucleotide 1858 (95% vs. 0%; P<.001), and a lower prevalence of precore stop codon mutations (5% vs. 50%; P = .002). Conclusions. HBV genotype C can be differentiated into 2 subgroups-namely, genotype Ce and genotype Cs-that have different epidemiological distributions and virological characteristics. © 2005 by the Infectious Diseases Society of America. All rights reserved.enMutationBase SequenceGenomeGenotypeHong KongHepatitis B VirusVirologyPeer Reviewed Journal Article10.1086/430324